Electron microscopic analysis of heat-induced leakage of polyphosphate from a phoU mutant of Escherichia coli.

نویسندگان

  • Hitoshi Kashihara
  • Bong Mun Kang
  • Takeshi Omasa
  • Kohsuke Honda
  • Yuka Sameshima
  • Akio Kuroda
  • Hisao Ohtake
چکیده

Heat treatment is a simple technique for releasing polyphosphate (polyP) from microbial cells. We investigated the ultrastructural alterations of polyP granules (PPGs) in response to heat treatment at 70 degrees C using a polyP-accumulating phoU mutant of Escherichia coli as a model organism. Electron microscopic and energy-dispersive X-ray analysis suggested concurrent occurrence of PPG degradation and polyP release in the cytoplasm. PolyP is probably released by cytoplasmic extrusion through breaks in the cell envelope.

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عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 74 4  شماره 

صفحات  -

تاریخ انتشار 2010